Coding
mGFP5

Part:BBa_K3183011:Design

Designed by: David Schramm   Group: iGEM19_Oxford   (2019-10-14)


Modified Green Fluorescent Protein, Codon Optimized for L. reuteri


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 644


Design Notes

RF10 Compatible


Source

https://www.ncbi.nlm.nih.gov/pubmed/8994830

The part was included in the pTRKH3 vector (Lizier et al.), which was purchased from Addgene

References

Lizier, Michela, et al. “Comparison of Expression Vectors in Lactobacillus Reuteri  Strains.” FEMS Microbiology Letters, vol. 308, no. 1, Aug. 2010, pp. 8–15., doi:10.1111/j.1574-6968.2010.01978.x.